ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (9): 1531-1537.doi: 10.11843/j.issn.0366-6964.2014.09.021

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Identification of SNPs in Myf5 and Pax7 Genes and Association Analysis with Body Measurement Traits in Xiangxi Cattle

WANG Xing-ping*,LUORENG Zhuo-ma,LI Feng,LI De-cai,DU Xin   

  1. (Key Laboratory of Zoology in Hunan Higher Education School,College of Life Science,Hunan University of Arts and Science,Changde 415000,China)
  • Received:2014-03-04 Online:2014-09-23 Published:2014-09-23

Abstract:

This research aimed to identify single nucleotide polymorphism (SNP) of Myf5 and Pax7 genes in Xiangxi cattle,investigate the population genetic characters of SNPs and search the molecular markers for growth.SNPs of intron 2 in Myf5 gene and exon 3 in Pax7 gene were detected by PCR and sequencing methods.Population genetic polymorphisms for 217 Xiangxi cattle were detected by PCR-RFLP,and the association analysis between genotypes and body measurement traits was performed by least-squares linear model.The results showed that the total of 9 SNPs were detected on intron 2 in Myf5 gene,in which,7 SNPs were novel.While,only one SNP was detected on exon 3 in Pax7 gene.The results of population genetic polymorphism indicated,for g.1948A>G locus of Myf5 gene in Xiangxi cattle,the genotype frequencies of AA,AG,GG were 0.046 1,0.235 0,0.718 9.Allele frequencies of A and G were 0.163 6 and 0.836 4,respectively.For g.31G>A locus of Pax7 gene,only two genotypes of AG and GG were detected in Xiangxi cattle,and the genotype frequencies were 0.073 7 and 0.926 3,the allele frequencies of A and G were 0.036 9 and 0.963 1,respectively.Both loci were low polymorphism,and fitted with Hardy-Weinberg equilibrium (P>0.05).The results of association analysis for g.1948A>G locus in Myf5 gene revealed that the animals with AA and AG genotypes had higher body weight than those with GG genotype in Xiangxi cattle (P<0.05),while there were not significant differences for withers height,body length and heart girth among the individuals with different genotypes (P>0.05).Allele A positively affected on body weight in Xiangxi cattle.These results indicated that there were 9 SNPs in Myf5 gene,in which,g.1948A>G locus might be molecular marker for body weight.

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